000			02772nam a2200241Ia 4500
003			NULRC
005			20250520102725.0
008			250520s9999 xx 000 0 und d
020			_a9780136043027
040			_cNULRC
050			_aQD 415.5 .B69 2012
100			_aBoyer, Rodney. _eauthor
245		0	_aBiochemistry laboratory: _bmodern theory and techniques / _cRodney Boyer.
250			_aSecond edition.
260			_aBoston, Massachusetts : _bPrentice-Hall, _cc2012
300			_axix, 362 pages : _billustrations ; _c24 cm.
365			_bUSD74.67
504			_aIncludes bibliographical references and index.
505			_aIntroduction to the biochemistry/molecular biology laboratory -- Using the computer and internet for research in biochemistry -- General laboratory procedures -- Centrifugation techniques in biochemistry/molecular biology -- Purification and analysis of biomolecules by chromatography -- Characterization of proteins and nucleic acids by electrophoresis -- Spectroscopic analysis of biomolecules -- Biomolecular interactions: ligand binding and enzyme reacations -- Molecular biology I: structures and analysis of nucleic acids -- Molecular biology II: recombinant DNA, molecular cloning and enzymology -- Protein production, purification, and characterization. Appendix I. List of software programs and web sites useful for each chapter -- Appendix II. Properties of common acids and bases -- Appendix III. Properties of common buffer compounds -- Appendix IV. pKa values and pHI values of amino acids -- Appendix V. Molecular weight of some common proteins -- Appendix VI. Common abbreviations used in this text -- Appendix VII. Units of measurement -- Appendix VIII. Table of the elements -- Appendix IX. Answers to odd-numbered study problems.
520			_aYour biochemistry lab course is an essential component in training for a career in biochemistry, molecular biology, chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Biochemistry Laboratory: Modern Theory and Techniques covers the theories, techniques, and methodologies practiced in the biochemistry teaching and research lab. Instead of specific experiments, it focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. An extensive range of techniques discussed includes Internet databases, chromatography, spectroscopy, and recombinant DNA techniques such as molecular cloning and PCR. The Second Edition introduces cutting-edge topics such as membrane-based chromatography, adds new exercises and problems throughout, and offers a completely updated Companion Website.
650			_aBIOCHEMISTRY -- LABORATORY MANUALS
942			_2lcc _cBK
999			_c13806 _d13806