Microbiology : laboratory manual / Russell Bey

Includes index.

Preface. General Laboratory Directions. Exercise 1: Ubiquity of Microorganisms. Exercise 2: Effectiveness of Hand Washing. Exercise 3: Microscopy. Exercise 4: Eukaryotes - The Larger Microorganisms. Part A: Fungi and Terrestrial Protozoa/ Part B: Algae/ Part C: Aquatic Protozoa. Exercise 5: Handling of Microorganisms. Part A: Slide Preparations/ Part B: Introduction to Staining. Exercise 6: Aseptic Transfer. Exercise 7: Pure Culture Isolation of Bacteria. Part A: Enrichment Culture/ Part B: The Streak-Plate Isolation. Exercise 8: Gross Anatomy of Bacteria. Part A: Flagella/ Part B: Endospores. Exercise 9: The Culture Medium. Part A: Sources of Carbon, Nitrogen and Energy/ Part B: Selective Media. Exercise 10: Growth Features of Bacteria and Fungi. Part A: Features of the Fungi/ Part B: Cultured Features of Bacteria. Exercise 11: Effects of Environment on Microorganisms. Part A: Oxygen/ Part B: Temperature. Exercise 12: Extracellular Hydrolytic Enzymes. Part A: Hydrolysis of Starch/ Part B: Casein Hydrolysis/ Part C: Hydrolysis of Gelatin. Exercise 13: Influence of High Temperatures on Bacteria. Exercise 14: Enumeration of Bacteria and Growth Curve. Exercise 15: Chemotactic and Phototropic Response to Environment. Part A: Chemotaxis in Bacteria/ Part B: Phototropism in Pilobolus. Exercise 16: Bacterial Transformation. Exercise 17: Drug Resistance Transfer Between Bacteria. Exercise 18: Viruses of Bacteria. Exercise 19: Destruction and Inhibition of Microorganisms. Part A: Antiseptics and Disinfectants/ Part B: Action of Antibiotics. Exercise 20: The Nitrogen-Fixing Bacteria. Exercise 21: Microbiological Examination of Milk. Part A: Agar Plate Count/ Part B: Coliform Count. Exercise 22: Microbiological Analysis of Food Products. Exercise 23: Sanitary Examination of Water. Part A: Examination of Water for Coliform Bacteria/ Part B: The ?IMViC? Test/ Part C: Demonstration of Membrane Filtration. Exercise 24: Action of Antibiotics. Part A: Antibiotic Production as Determined Qualitatively by the Cross-Streak Plate Method/ Part B: Paper Disc Method for Testing Antibiotic Sensitivity. Exercise 25: Serological Reactions. Exercise 26: The Pathogenic Cocci. Exercise 27: The Gram-Positive Pathogenic Rods. Part A: Gram-Positive Endospore-Forming Rods/ Part B: Gram-Positive Nonsporing Rods. Exercise 28: The Fermentative Gram-Negative Rods. Exercise 29: Identification of Unknowns. Exercise 30: Group Project.

This manual contains a carefully selected set of lab exercises covering both classic and state-of-the-art experiments, with an emphasis on the development of critical-thinking skills and an understanding of the scientific method. It offers the opportunity to implement innovative, engaging, and effective labs, which have been class-tested for years.