Laboratory exercises in organismal and molecular microbiology /
Alexander, Steve K.
Laboratory exercises in organismal and molecular microbiology / Steve K. Alexander, Dennis Strete and Mary Jane Niles. - Boston, Massachusetts : McGraw Hill Education, c2004 - xiii, 362 pages : color illustrations ; 28 cm
Includes index.
Safety in the Microbiology Laboratory I Survey of Microscopic Organisms A The Microscope 1 Structure, Function, and Use of the Microscope 2 Microscopic Comparisons of Microorganisms, PMulticellular arasites, and Microscopic Invertebrates B Microorganisms 3 Microbial Prokaryotes: Bacteria and Cyanobacteria 4 Microbial Eucaryotes: Fungi 5 Microbial Eucaryotes: Protozoans and Algae C Multicellular Parasites 6 Flatworms and Roundworms D Microscopic Invertebrates 7 Zooplankton 8 Disease Vectors II Staining Techniques A Morphological Stains 9 Negative Stain 10 Smear Preparation and the Simple Stain B Differential Stains 11 Gram Stain 12 Acid-fast Stain C Structural Stains 13 Spore Stain 14 Capsule and Flagella Stains D Application 15 The Staining Characterization of a Bacterial Unknown III Bacterial Cultivation A Aseptic Technique 16 Bacteria and Fungi in the Laboratory Environment: The Necessity of Aseptic Technique B Culture of Bacteria 17 Preparation and Inoculation of Growth Media 18 Culture Characterization of Bacteria IV Bacterial Identification 19 Biochemical Tests Used to Identify Bacteria 20 Application: The Identification of a Nonclinical Bacterial Unknown V Medical Microbiology A Bacteria of the Human Body 21 Isolation and Identification of Staphylococci from the Skin 22 Isolation and Identification of Streptococci from the Throat 23A Identification of Enteric Bacteria, Including the Intestinal Pathogens Salmonella and Shigella 23B Enterotubes, a Rapid Test System to Identify Enteric Bacteria 24 Isolation and Identification of Bacteria from the Urinary Tract B Treatment of Bacterial Infections 25 Assessing Antibiotic Effectiveness: The Kirby-Bauer Method C Application 26 Identification of a Clinical Bacterial Unknown VI Controlling the Risk and Spread of Bacterial Infections A Proper Food Handling 27 Killing Bacteria with High Temperature B Disinfecting Skin and Countertops 28 Skin Disinfection: Evaluating Antiseptics and Hand Sanitizers 29 Cleaning Countertops with Disinfectants C Testing Drinking Water Safety 30 Bacteriological Examination of Drinking Water Using the MPN Method VII Bacterial Genetics A Bacterial Genomic DNA 31 Bacterial DNA Isolation and Southern Analysis 32 Mutagenesis in Bacteria: The Ames Test B Plasmid DNA 33 Plasmid Isolation and Restriction Mapping C The Transfer of Drug Resistance 34 Acquiring Antibiotic Resistance Through Bacterial Transformation 35 Acquiring Antibiotic Resistance Through Bacterial Conjugation VIII Viruses A The Nature and Characterization of Viruses 36 Viral DNA Fingerprinting B Virus Propagation 37 Isolation of Bacteriophage from Sewage and Determination of Phage Titer 38 The Virus Infection Cycle: The One-step Growth Curve 39 Infection of Plant Leaves with Tobacco Mosaic Virus IX Hematology and Serology A Hematology 40 Identification and Enumeration of White Blood Cells B Serology 41 Antigen-Antibody Precipitation Reactions and Determination of Antibody Titer C Serological Techniques 42 Agglutination Reactions: ABO Blood Typing 43 Immunodiffusion: Antigen-Antibody Precipitation Reactions in Gels 44 Enzyme-Linked Immunosorbent Assay (ELISA) 45 Bacterial Protein Fingerprinting and Western Blotting 46 The Neutralization of Viruses by Antibodies
A modern general microbiology laboratory manual that combines the procedural details of a laboratory manual with the photographic support of a laboratory atlas. The 46 class-tested laboratory experiments are divided into 9 specialty areas, and the extensive four-color illustration program includes 220 photos and micrographs plus 150 line drawings.
72487445
MICROBIOLOGY
QR 63 .A44 2004
Laboratory exercises in organismal and molecular microbiology / Steve K. Alexander, Dennis Strete and Mary Jane Niles. - Boston, Massachusetts : McGraw Hill Education, c2004 - xiii, 362 pages : color illustrations ; 28 cm
Includes index.
Safety in the Microbiology Laboratory I Survey of Microscopic Organisms A The Microscope 1 Structure, Function, and Use of the Microscope 2 Microscopic Comparisons of Microorganisms, PMulticellular arasites, and Microscopic Invertebrates B Microorganisms 3 Microbial Prokaryotes: Bacteria and Cyanobacteria 4 Microbial Eucaryotes: Fungi 5 Microbial Eucaryotes: Protozoans and Algae C Multicellular Parasites 6 Flatworms and Roundworms D Microscopic Invertebrates 7 Zooplankton 8 Disease Vectors II Staining Techniques A Morphological Stains 9 Negative Stain 10 Smear Preparation and the Simple Stain B Differential Stains 11 Gram Stain 12 Acid-fast Stain C Structural Stains 13 Spore Stain 14 Capsule and Flagella Stains D Application 15 The Staining Characterization of a Bacterial Unknown III Bacterial Cultivation A Aseptic Technique 16 Bacteria and Fungi in the Laboratory Environment: The Necessity of Aseptic Technique B Culture of Bacteria 17 Preparation and Inoculation of Growth Media 18 Culture Characterization of Bacteria IV Bacterial Identification 19 Biochemical Tests Used to Identify Bacteria 20 Application: The Identification of a Nonclinical Bacterial Unknown V Medical Microbiology A Bacteria of the Human Body 21 Isolation and Identification of Staphylococci from the Skin 22 Isolation and Identification of Streptococci from the Throat 23A Identification of Enteric Bacteria, Including the Intestinal Pathogens Salmonella and Shigella 23B Enterotubes, a Rapid Test System to Identify Enteric Bacteria 24 Isolation and Identification of Bacteria from the Urinary Tract B Treatment of Bacterial Infections 25 Assessing Antibiotic Effectiveness: The Kirby-Bauer Method C Application 26 Identification of a Clinical Bacterial Unknown VI Controlling the Risk and Spread of Bacterial Infections A Proper Food Handling 27 Killing Bacteria with High Temperature B Disinfecting Skin and Countertops 28 Skin Disinfection: Evaluating Antiseptics and Hand Sanitizers 29 Cleaning Countertops with Disinfectants C Testing Drinking Water Safety 30 Bacteriological Examination of Drinking Water Using the MPN Method VII Bacterial Genetics A Bacterial Genomic DNA 31 Bacterial DNA Isolation and Southern Analysis 32 Mutagenesis in Bacteria: The Ames Test B Plasmid DNA 33 Plasmid Isolation and Restriction Mapping C The Transfer of Drug Resistance 34 Acquiring Antibiotic Resistance Through Bacterial Transformation 35 Acquiring Antibiotic Resistance Through Bacterial Conjugation VIII Viruses A The Nature and Characterization of Viruses 36 Viral DNA Fingerprinting B Virus Propagation 37 Isolation of Bacteriophage from Sewage and Determination of Phage Titer 38 The Virus Infection Cycle: The One-step Growth Curve 39 Infection of Plant Leaves with Tobacco Mosaic Virus IX Hematology and Serology A Hematology 40 Identification and Enumeration of White Blood Cells B Serology 41 Antigen-Antibody Precipitation Reactions and Determination of Antibody Titer C Serological Techniques 42 Agglutination Reactions: ABO Blood Typing 43 Immunodiffusion: Antigen-Antibody Precipitation Reactions in Gels 44 Enzyme-Linked Immunosorbent Assay (ELISA) 45 Bacterial Protein Fingerprinting and Western Blotting 46 The Neutralization of Viruses by Antibodies
A modern general microbiology laboratory manual that combines the procedural details of a laboratory manual with the photographic support of a laboratory atlas. The 46 class-tested laboratory experiments are divided into 9 specialty areas, and the extensive four-color illustration program includes 220 photos and micrographs plus 150 line drawings.
72487445
MICROBIOLOGY
QR 63 .A44 2004